•   908-769-5555
  •   info@accuris-usa.com
  • QuickSilver Powdered Buffer Packs

    QUICK , CONVENIENT, and ECONOMICAL

    Accuris QuickSilver™ Powdered Buffer Packs … Fresh buffer, when you need it.

    • Premeasured pouches make 1 liter of 1X buffer solution
    • Just empty pouch into water and stir – no diluting of stock solutions
    • No pH adjustment necessary
    • Quality and consistency guaranteed
    • Nuclease and protease free
    • Long shelf life

    Electrophoresis Buffers:

    TBE,  TAE,  Fast Running Buffer,  Tris-Glycine-SDS,  Tris-MOPS-SDS,  Tris-MES-SDS

    Western Blotting, ELISA and Cell Culture:

    TBS, TBST, PBS, PBST

    Nucleic Acid Electrophoresis Buffers

    QuickSilver™ TAE (Tris-acetate-EDTA)

    TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.

    TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.

    EB1000 QuickSilver TAE Buffer, 1X, 100 packs

    QuickSilver™ TBE (Tris-borate-EDTA)

    TBE is used in gel preparation and as a running buffer for electrophoresis of nucleic acids. TBE works best for resolution of smaller fragments (<2kb). Its higher buffering capacity makes it useful for longer runs. The final concentration of a 1X working solution is 89mM Tris, 89mM boric acid, 2mM EDTA.

    EB1001 QuickSilver TBE Buffer, 1X, 50 packs

    QuickSilver™ Fast Running Buffer

    QuickSilver Fast Running Buffer is a proprietary blend formulated for agarose gel electrophoresis at high voltage (300-350V). Its strong buffering capacity minimizes heat generation all allows for extremely fast separation of fragments smaller that 5kb. A typical 30 minute run can be performed in just 10 minutes using this buffer.

    EB1002 QuickSilver Fast Running Buffer, 1X, 100 packs

    Protein Electrophoresis Buffers

    QuickSilver™ TGS (Tris-Glycine-SDS)

    Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.

    EB1100 QuickSilver Tris Glycine-SDS Buffer, 1X, 50 packs

    QuickSilver™ Tris-MES-SDS

    Small to medium molecular weight proteins can be resolved on SDS-PAGE gels using Tris-MES-SDS as a running buffer. Proteins resolve faster in gels using Tris-MES-SDS as a running buffer than those using Tris-MOPS-SDS. A 1X solution has a final concentration of 50mM Tris, 50mM MES, 0.1% SDS and 1mM EDTA.

    EB1101 QuickSilver Tris-MES-SDS Buffer, 1X, 50 packs

    QuickSilver™ Tris-MOPS-SDS

    Tris-MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins with SDS-PAGE. Gels run with Tris-MOPS-SDS buffer run slower than those using Tris-MES-SDS buffer. The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and 1mM EDTA.

    EB1102 QuickSilver Tris-MOPS-SDS, 1X, 50 packs

    Western Blotting, ELISA, Cell Culture, Chromatography

    QuickSilver™ PBS and PBST

    Because its osmolality and ion concentration closely mimic that of the human body, PBS is commonly used for cell culture applications, as well as HPLC and MS. PBST is a wash buffer and diluent for ELISA. The 1X PBS solution contains 137mN NaCl, 2.7mM KCl and 10mM phosphate. PBST is supplemented with 0.05% Tween-20.

    EB1200 QuickSilver PBS Buffer, 1X, 100 packs

    EB1201 QuickSilver PBST Buffer, 1X, 100 packs

    QuickSilver™ TBS and TBST

    TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.

    EB1202 QuickSilver TBS buffer, 1X, 100 packs

    EB1203 QuickSilver TBST buffer, 1X, 100 packs

     

    Ordering Information:

    Item No.Description
    EB1000QuickSilver TAE Buffer Powder, 100 pouches
    EB1001QuickSilver TBE Buffer Powder, 50 pouches
    EB1002QuickSilver Fast Running Buffer Powder, 100 pouches
    EB1100QuickSilver Tris-Glycine-SDS Buffer Powder, 50 pouches
    EB1101QuickSilver Tris-MES-SDS Buffer Powder, 50 pouches
    EB1102QuickSilver Tris-MOPS-SDS Buffer Powder, 50 pouches
    EB1200QuickSilver PBS Buffer Powder, 100 pouches
    EB1201QuickSilver PBS-T Buffer Powder, 100 pouches
    EB1202QuickSilver TBS Buffer Powder, 100 pouches
    EB1203QuickSilver TBS-T Buffer Powder, 100 pouches